Changes within genes are considered a random and relatively slow process, somewhat speeded up by the presence of mutagens. Beneficial evolutionary changes are selected over generations when mutants show advantageous behavior and win in competition in changing environments.
The impact of the Trivedi Effect, however,is a natural adaptation guided to the target by human intention, seen at the genetic level and expressing itself as an all-round improvement in every parameter studied, with a lush growth and vitality glow in the plants under observation. These changes are true adaptive responses as proved by growth studies, and are true genetic changes as seen in DNA polymorphism tests (i.e. DNA comparison tests), which have also been found to remain stable through succeeding generations.
The Trivedi Effect has also been studied at the level of microbial genetics, where it has proven impact on antibiotic susceptibilities and genetic characteristics of microbes.
Experiments in Plant Genetics
Seeds, plants, as well as plant tissue cultures when treated to the Trivedi Effect showed statistically significant improvements in growth, immunity, yield and many other parameters (see Agriculture section).
These improvements were normally accompanied by clearly visible changes in the appearance of the plant, i.e. its morphology and expression. Changes are seen in the shape, size and texture of leaves or stems as well as clustering, branching and inflorescence patterns.
These visual indications of genetic changes were verified through DNA fingerprinting tests on over 40 plant species in two different laboratories.
Two series of seed aliquots were prepared, one as control and the other briefly treated by Guruji for about 3 minutes. The sets were sowed and leaf discs harvested for DNA extraction when the plants reached the appropriate stage.
Two different kinds of DNA fingerprinting tests were used: RAPD analysis at Bangalore, India, and SSR markers at Montreal, Canada. The reports can be seen by clicking on the links provided below for each set.
DNA Polymorphism refers to different DNA forms (called different ‘alleles’) within the same species, which can show up as visible differences between members of the same species. True polymorphism is when the change in DNA is not a minor mutation but large enough to be a real change in the same species. There can be other types of changes causing lesser mutations. For example, changes in molecular weight can occur when the DNA loses a section through fragmentation or when it gains one, which is a mutation but may not be a change causing an allelic variation.
38 species of vegetables or staple crops were tested in December 2007 at Bangalore Genei (India) through DNA fingerprinting by RAPD analysis using 5 to 8 markers depending on the crop species.
DNA polymorphism was seen in all the 38 samples, ranging from 7.8% in sponge gourd to 69% in yellow pigeon peas. Although the variation in the sponge gourd was relatively less than in others in these lab experiments, in the field in one case the gourd has been seen to change from a creeper to an erect plant for upto 6 weeks following the treatment, showing a major gain in vitality.
3 crop species were earlier tested in May 2006 at DNA Landmark, Montreal (Canada) with DNA fingerprinting tests using 100 SSR markers for each: Canola, Tomato and Cotton.
In all the three species studied polymorphism was clearly seen, which could be classified into two categories: the first, a true allelic variation and the second an additional band or shift in molecular weight.True polymorphism of 15.3% was seen in Canola and 4.0% in cotton.
In July 2002, 2 varieties of Cashew crops (V4 & V7) were planted on treated plots and DNA fingerprinting tests using 5 markers each were done in March 2008 at Bangalore Genei (India). True polymorphism upto 55% was observed in both varieties when compared with the controls and also when different treated plants of a particular variety were compared within themselves.
On the other hand, substantial improvements observed in Mango trees, including change in inflorescence patterns and male-female dominance of flowers, were not accompanied by any genetic changes, showing that improvements in vitality may be sufficient to powerfully improve competitive and self-expressive abilities without necessarily involving changes at genetic levels.
Bacteria treated by the Trivedi Energy showed substantial changes in their antibiotic susceptibilities and responses to chemical reactions, tests which are used to characterize and identify them. Changes seen included up to
alteration in the genotype itself (for more details, see Microbiology section) These observations were re-tested using DNA fingerprinting tests by RAPD analysis on 6 samples, by using the highly rigorous 16 S rDNA sequence-based identification test on 10 samples, and by using both tests on 5 samples.
The tests confirmed true DNA polymorphism of 32% to 79% between control and treated bacteria, while 40% of the samples examined by 16 S rDNA tests showed changes in the genotype also.
Reports may be seen at the pages given below:
Click link to see Report